Jun Zhang, M.S.
Graduate Student and Research Associate
Bachelor of Science, Microbiology
Shandong University, 1991

Master of Science, Biochemistry
Chinese Academy of Sciences, 1994.

e-mail: Julyjun@yahoo.com

Research interest:

My research has focussed on the functional proteomic characterization of the PKCe signaling system in the heart. Over the past 4 years, I have used two dimensional electrophoresis, mass spectrometry, immunoprecipitation, and a host other of classical biochemical and molecular biological approaches to characterize PKC complexes in the normal and protected heart. Furthermore, I am currently focussing my dissertation research project on the extensive functional characterization of a specific module within these complexes. In addition, I am in the process of developing novel techniques to analyze native protein complexes.

Publications:

1. Edmondson RD, Vondriska TM, Biederman KJ, Zhang J, Jones RC, Zheng YT, Allen DL, Xiu JX, Cardwell EM, Pisano MR, Ping P. Protein kinase Ce _signaling complexes include metabolism-and transcription/translation-related proteins. Mol Cell Proteomics. 2002,1:421-433.

2. Baines CP, Zhang J, Wang GW, Zheng YT, Xiu JX, Cardwell EM, Bolli R, Ping P. Mitochondrial PKC epsilon and MAPK form signaling modules in the murine heart: enhanced mitochondrial PKC epsilon-MAPK interactions and differential MAPK activation in PKC epsilon-induced cardioprotection. Circ Res. 2002;90:390-397.

3. Ping P, Song C, Zhang J, Guo Y, Cao X, Li RC, Wu W, Vondriska TM, Pass JM, Tang XL, Pierce WM, Bolli R. Formation of protein kinase C(epsilon)-Lck signaling modules confers cardioprotection. J Clin Invest. 2002;109:499-507.

4. Balafanova Z, Bolli R, Zhang J, Zheng Y, Pass JM, Bhatnagar A, Tang XL, Wang O, Cardwell E, Ping P. Nitric oxide induces nitration of PKC epsilon, facilitating PKC epsilon translocation via enhanced PKC epsilon-RACK2 interactions: A novel mechanism of NO-triggered activation of PKC epsilon. J Biol Chem. 2002;277:15021-15027.

5. Song C, Vondriska TM, Wang GW, Klein JB, Cao X, Zhang J, Kang YJ, D'Souza S, Ping P. Molecular conformation dictates signaling module formation: example of PKC epsilon and Src tyrosine kinase. Am J Physiol. 2002;282:H1166-71.

6. Pass JM, Gao J, Jones WK, Wead WB, Wu X, Zhang J, Baines CP, Bolli R, Zheng YT, Joshua IG, Ping P. Enhanced PKC beta II translocation and PKC beta II-RACK1 interactions in PKC epsilon-induced heart failure: a role for RACK1 Am J Physiol. 2001;281:H2500-10.

7. Zhang J, Vondriska TM, Song C, Tang XL, Cao X, Baines CP, Pass JM, Wang S, Bolli R, Ping P. Protein kinase C epsilon-Src modules direct signal transduction in nitric oxide-induced cardioprotection: complex formation as a means for cardioprotective signaling. Circ Res. 2001;22;88:1306-13.

8. Pass JM, Zheng Y, Wead WB, Zhang J, Li RC, Bolli R, Ping P. PKC epsilon activation induces dichotomous cardiac phenotypes and modulates PKC epsilon-RACK interactions and RACK expression. Am J Physiol. 2001;280:H946-55.

9. Ping P, Zhang J, Pierce WM Jr, Bolli R. Functional proteomic analysis of protein kinase C epsilon signaling complexes in the normal heart and during cardioprotection. Circ Res. 2001;88:59-62.

10. Li RCX, Ping P, Zhang J, Wead WB, Cao X, Gao J, Zheng Y, Huang S, Han J, Bolli R. PKC epsilon modulates NF-kappa B and AP-1 via mitogen-activated protein kinases in adult rabbit cardiomyocytes. Am J Physiol. 2000;279:H1679-89.

11. Ping P, Zhang J, Huang S, Cao X, Tang XL, Li RC, Zheng YT, Qiu Y, Clerk A, Sugden P, Han J, Bolli R. PKC-dependent activation of p46/p54 JNKs during ischemic preconditioning in conscious rabbits. Am J Physiol. 1999;277:H1771-85.

12. Ping P, Zhang J, Zheng YT, Li RCX, Dawn B, Tang XL, Takano H, Balafanova Z, Bolli R. Demonstration of selective protein kinase C-dependent activation of Src and Lck tyrosine kinases during ischemic preconditioning in conscious rabbits. Circ Res. 1999;85:542-50.

13. Ping P, Zhang J, Cao X, Li RCX, Kong D, Tang XL, Qiu Y, Manchikalapudi S, Auchampach JA, Black RG, Bolli R. PKC-dependent activation of p44/p42 MAPKs during myocardial ischemia-reperfusion in conscious rabbits. Am J Physiol. 1999;276:H1468-81.

14. Ping P, Takano H, Zhang J, Tang XL, Qiu Y, Li RCX, Banerjee S, Dawn B, Balafonova Z, Bolli R. Isoform-selective activation of protein kinase C by nitric oxide in the heart of conscious rabbits: a signaling mechanism for both nitric oxide-induced and ischemia-induced preconditioning. Circ Res. 1999;84:587-604.

15. Bolli R, Dawn B, Tang XL, Qiu Y, Ping P, Xuan YT, Jones WK, Takano H, Guo Y, Zhang J. The nitric oxide hypothesis of late preconditioning. Basic Res Cardiol. 1998;93:325-38.

16. Qiu Y, Ping P, Tang XL, Manchikalapudi S, Rizvi A, Zhang J, Takano H, Wu WJ, Teschner S, Bolli R. Direct evidence that protein kinase C plays an essential role in the development of late preconditioning against myocardial stunning in conscious rabbits and that epsilon is the isoform involved. J Clin Invest. 1998;101:2182-98.

17. Ping P, Zhang J, Qiu Y, Tang XL, Manchikalapudi S, Cao X, Bolli R. Ischemic preconditioning induces selective translocation of protein kinase C isoforms epsilon and eta in the heart of conscious rabbits without subcellular redistribution of total protein kinase C activity. Circ Res. 1997;81:404-14.

18. Zhang J, Xu JL. Oleyl oleate synthesis by immobilized lipase from Candida sp.-1619. Chinese J. of Biotechnology.1995;11:243-252.

19. Xu JL, Zhang J. Studies on the lipase from Penicillium Camembertii-PG3. Acta Mycologica Sinica. 1995;14:136-142.

20. Zhang J, Xu JL. Oleyl oleate synthesis by immobilized lipase from Candida sp.-1619. Chinese Journal of Biotechnology. 1995;11:325-331.