| Core
Overview: |
The
Proteomic Core was formed on the basis of collaborative proteomics
investigations already in progress at UCLA in the laboratories of
the Project Leaders. A central objective of the Core is to
consolidate proteomics expertise and resources to facilitate data
acquisition, mining and interpretation in each Project by providing
state-of-the-art technology platforms to test individual
hypotheses.
The Proteomic Core will play a
leadership role in achieving our central objectives to mapping four
subproteomes underlying the manifestation of injurious or protective
phenotype. Thus, the primary goal of the Proteomic Core is to
facilitate testing of our central hypothesis by making available
state-of-the-art proteomics applications to all members of the PPG.
Accordingly,
each of the individual Projects will utilize the resources of the
Proteomic Core in their specific aims. These subproteome mapping
studies are critical components of each individual Project and serve
as hypothesis-testing as well as hypothesis-generating experiments.
Also, all Project Leaders have proposed, in consultation with the
Proteomic Core, extensive additional studies to verify the
functional significance of candidate interacting partners identified
by these analyses. Thus, the collaborative studies conducted with
the Proteomic Core will be integral parts of each of the four
Projects. In general, the Proteomic Core will interact with all
Projects to aid with sample preparation and quality control before
and during proteomic experiments, and with interpretation of data
afterwards. Upon completion of initial protein identification
experiments, the Proteomic Core will facilitate the process of
target validation in collaboration with the Heart Biology Core. In
this regard, the Proteomic Core serves the central theme of the PPG
by enabling subproteomic information to be obtained and linked to
myocardial phenotype.
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